The best Side of stem cell clinic
The best Side of stem cell clinic
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Tightly packed monolayers would require some trituration to develop one-cell suspensions. Steer clear of excessive trituration or shear force by resting the pipette idea towards the plate edge to keep up viability. If cells never dissociate perfectly, incubation time with dissociation reagent may should be optimized.
Very best effects are obtained when limiting extended cell publicity to ambient temperature conditions. Contemplate holding unused cells inside a humidified incubator with 5% CO2 at 37°C when executing much larger experiments.
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Be aware: Commence to antibody staining if accomplishing live cell Examination following this phase. Alternatively, progress to fixable dye staining and PFA fixation.
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*This can be a grasp materials checklist to the underneath protocols—all reagents pointed out in Each and every protocol are summarized.
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Get rid of supernatant and resuspend cells in FACS buffer. Note: It is important to quench the dissociation reagent by utilizing the identical or double the volume with the dissociation reagent.